In our InSpector-01 tool, we characterize the metabolic activity of the causative pathogen. We can do the same measurement with a varying amount of antimicrobial. The result is a rapid characterization of both MIC (Minimum Inhibitory Concentration MIC) and MBC (Minimum Bactericidal Concentration) of the candidate antimicrobial.
For this, we do need a small period of incubation (about 4 hours) from the blood draw. The initial blood draw has about 1-10 copies of the bacteria. We need to incubate this in a rich media until we have about 500 copies of the bacteria (this will take 3-4 hours). At that point, we aliquot the sample into multiple parts, add the desired antimicrobial, wait for 20 minutes, and test (we can use the same reagent and hardware as in InSpector-01).
This tool is now ready for clinical testing. We anticipate that clinical testing will also entail some changes to the hardware/reagent/software, and that it will be ready for discussion with the FDA in 2020.